High peroxidase-like activity of metallic cobalt nanoparticles encapsulated in metal–organic frameworks derived carbon for biosensing
| Autor: | Yunxia Zhuang, Hongxiang Chai, Siqi Li, Xiaodan Zhang, Yuming Huang, Wenfei Dong |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Analytical chemistry
chemistry.chemical_element Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences Metal Materials Chemistry Glucose oxidase Electrical and Electronic Engineering Instrumentation Detection limit biology Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry visual_art visual_art.visual_art_medium biology.protein Metal-organic framework 0210 nano-technology Cobalt Biosensor Carbon Nuclear chemistry |
| Zdroj: | Sensors and Actuators B: Chemical. 255:2050-2057 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.09.013 |
| Popis: | Encapsulating metal nanoparticles (NPs) into carbon materials is a promising strategy for the fabrication of nanozymes with high activity. Here, for the first time, the proof-of-concept is demonstrated by fabricating CoNPs embedded in NH2-MIL-88(Fe) MOFs-derived magnetic carbon (MC) through in situ reduction of cobalt precursors by NaBH4. The obtained CoNPs/MC was characterized by TEM, SEM, XPS, XRD, Raman spectrum, and FT-IR. The CoNPs/MC exhibits much higher peroxidase-like activity than pure CoNPs and magnetic carbon. The high peroxidase-like activity of the CoNPs/MC is attributed to the decomposition of H2O2 to generate OH and O2 − radicals, resulting in TMB color reaction. Also, CoNPs/MC is robust in a wide range of pH and temperature, indicating a promising peroxidase-like candidate. On this basis, a colorimetric assay was developed by combining glucose oxidase with CoNPs/MC for glucose biosensing. The linear range for glucose detection is from 0.25 to 30 μM with a lower detection limit of 156 nM. The CoNPs/MC-based colorimetric method has been successfully used to assay the content of glucose in real biological samples such as human serums. Our work reveals the high potential of encapsulating metal NPs into carbon materials for the fabrication of nanozymes with stable and high activity. |
| Databáze: | OpenAIRE |
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