Fluorometric analysis of heat-stable alkaline phosphatase activity in human serum by means of high-performance liquid chromatography

Autor:	Norio Ichinose, Nobuhiko Nishino, Shozo Baba, Tadako Nakatsuji
Rok vydání:	1993
Předmět:	Detection limit chemistry.chemical_compound Chromatography chemistry Coefficient of variation Naphthol AS Fluorescence spectrometry Acetone Alkaline phosphatase Phosphate Biochemistry High-performance liquid chromatography Analytical Chemistry
Zdroj:	Fresenius' Journal of Analytical Chemistry. 345:610-612
ISSN:	1618-2650 0937-0633
DOI:	10.1007/bf00325810
Popis:	A method is proposed for the determination of heat-stable alkaline phosphatase (HSAP) in serum. It comprises the following steps. The serum is heat-inactivated at 65°C and the residual alkaline phosphatase is allowed to react with Naphthol AS-MX phosphate. After stopping the reaction by acetone, the liberated Naphthol AS-MX is measured fluorometrically using high-performance liquid chromatography (HPLC). This method can be applied to cancer-screening in clinical examination. It is sensitive and rapid as compared with existing methods. Linearity, accuracy and detection limit have been examined (linearity 0–2190 ng Naphthol AS-MX; coefficient of variation ≈7%; recovery ≈80%).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1c1e28d0e60796efe7d31f17b121b225 https://doi.org/10.1007/bf00325810 Zobrazit plný text záznamu Full text from SpringerLink