Antibody Islet Forming-Cells: Localized Hemolysis Inhibition in Gel
| Autor: | John S. Hege, Wallace V. Epstein |
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| Rok vydání: | 1972 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 108:1162-1171 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.108.5.1162 |
| Popis: | Spleen cells from mice immunized with human γ globulin (HGG) and goat erythrocytes coated with human Forssman hemolysin were imbedded in agar and incubated with complement. Generalized hemolysis occurred revealing islets of intact erythrocytes representing single spleen cells releasing anti-HGG antibody. Back-ground hemolysis was increased and islets were more distinct when the goat erythrocytes were incubated with a carbodiimide reagent before coating with hemolysin. Secondary HGG immunization yielded 50-fold more islets than did primary immunization. When background hemolysis was incomplete both islets and hemolytic plaques could be observed. Human IgM immunization yielded many more islets, but fewer plaques, than did IgG immunization. Evidence from this work and from the works of others suggests that islet-forming cells release antibody directed specifically against human immunoglobulin M, while plaque formation is the result of IgG antibody directed against human immunoglobulin G. In a system in which anti-globulin antibody modulates immune hemolysis, the immunoglobulin classes of both antiglobulin and hemolysin determine whether islets or plaques will occur. |
| Databáze: | OpenAIRE |
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