Adsorption of insulin on solid surfaces in relation to the surface properties of the monomeric and oligomeric forms
| Autor: | Paula Nilsson, Tommy Nylander, Svend Havelund |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Insulin
medicine.medical_treatment Oligomer Surfaces Coatings and Films Electronic Optical and Magnetic Materials Biomaterials Hydrophobic effect chemistry.chemical_compound Colloid and Surface Chemistry Adsorption Monomer chemistry Chemical engineering Ionic strength medicine Organic chemistry Wetting Protein adsorption |
| Zdroj: | Journal of Colloid and Interface Science. 144:145-152 |
| ISSN: | 0021-9797 |
| DOI: | 10.1016/0021-9797(91)90244-3 |
| Popis: | Adsorption of insulin on clean (hydrophilic) silica, methylated (hydrophobic) silica, and silica with a wettability gradient was studied by in situ ellipsometry. Both human and modified (monomeric) insulin were used. The adsorption was related to the surface properties of the different oligomeric forms of insulin. Adsorption of insulin, carrying a negative net charge, on the hydrophilic, negatively charged silica surface is dependent on the concentration, ionic strength, and type of ions added. No adsorption of monomeric insulin on the hydrophilic surface was observed. It is suggested that it is the hexameric form of insulin that adsorbs to the hydrophilic surface and that the adsorption is governed by electrostatic forces. Adsorption of insulin on the hydrophobic surface is independent of the concentration and, to a large extent, independent of the ionic strength and type of ions added. The amounts adsorbed of human insulin and monomeric insulin are the same on the hydrophobic surface. There is strong evidence that it is the monomeric form of insulin that adsorbs to the hydrophobic surface and that the driving force is hydrophobic interaction. |
| Databáze: | OpenAIRE |
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