Biodeterioration of mortar coating in historical buildings: Microclimatic characterization, material, and fungal community
Autor: | Denise Carpena Coitinho Dal Molin, Marilene Henning Vainstein, Fatima Menezes Bento, Juciana Clarice Cazarolli, Fernanda Lamego Guerra, Angela Borges Masuero, Mariane Rodrigues Lobato, Augusto Schrank, William Lopes |
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Rok vydání: | 2019 |
Předmět: |
Lecanicillium
Environmental Engineering Aggregate (composite) Moisture biology Geography Planning and Development 0211 other engineering and technologies Microclimate 02 engineering and technology Building and Construction 010501 environmental sciences engineering.material biology.organism_classification 01 natural sciences Horticulture Coating engineering Environmental science Relative humidity 021108 energy Mortar 0105 earth and related environmental sciences Civil and Structural Engineering Cladosporium |
Zdroj: | Building and Environment. 155:195-209 |
ISSN: | 0360-1323 |
DOI: | 10.1016/j.buildenv.2019.03.017 |
Popis: | The estimation of climate conditions and different moisture sources associated with the substrate composition and features in historical buildings is crucial to evaluate the substrate biodeterioration by filamentous fungi. Knowing the main agents and their frequency in substrates is relevant to propose conservation approaches focused on buildings preservation. Therefore, the mineralogic composition characterization of the historic building mortar coatings in Porto Alegre, RS was performed by binder/aggregate proportioning, x-ray diffraction, x-ray fluorescence, and thermogravimetric analyses. The microclimate conditions of indoor environments were evaluated through relative humidity (RH) and temperature recording sensors measurements. The qualitative and quantitative identification of filamentous fungi from the mortar coating was performed by microculture and DNA extraction for PCR and molecular analysis. The analyses indicated a mortar coating predominated by CaCO3 and MgCO3 with quartz sand and traces ratio between 1:2 and 1:5.4. The indoor microclimate had mean RH above 70% and a mean temperature above 20°C most of the time. Approximately 60 colonies of fungi were isolated, and 13 isolates were identified as the most frequent ones; among them: Aspergillus niger, Cladosporium sphaerospermum, Cladosporium spp., Purpureocillium lillacinus, Lecanicillium spp., Trichoderma atroviride, and T. harzianum. |
Databáze: | OpenAIRE |
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