Itraconazole encapsulated PLGA-nanoparticles covered with mannose as potential candidates against leishmaniasis
| Autor: | Mônica Pereira Garcia, Laura Fernanda Neira Fuentes, Ricardo Bentes Azevedo, Patricia Escobar, Jaqueline Rodrigues da Silva, Angélica M. Vera, Lubhandwa Sebastian Biswaro |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
Materials science Itraconazole Biomedical Engineering Mannose macromolecular substances 02 engineering and technology Biomaterials Plga nanoparticles 03 medical and health sciences chemistry.chemical_compound medicine Amastigote 030304 developmental biology 0303 health sciences biology technology industry and agriculture Leishmaniasis 021001 nanoscience & nanotechnology medicine.disease Leishmania biology.organism_classification Molecular biology PLGA chemistry Toxicity 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 107:680-687 |
| ISSN: | 1552-4973 |
| DOI: | 10.1002/jbm.b.34161 |
| Popis: | Leishmaniasis is a neglected disease threatening over 350 million people. Antimonials are first-line drugs due to resistance and side effects there is a demand for alternative chemotherapy. Itraconazole (ITZ) is an antimycotic. It was encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and covered with mannose. The NPs were 250 nm and -1.1 mV ± 0.7. PLGA-ITZ-mannose NPs presented a toxicity of 20.7% for J774 cells, and no toxicity for THP 1. The J774 cells were infected with three Leishmania promastigotes strains and treated with ITZ loaded PLGA NPs with/without mannose. The parasite percentage of L.(V.) panamensis intracellular amastigotes significantly (p < 0.01) decreased from 34.4% to 13.7% and 5.7% for PLGA-ITZ-mannose NPs and PLGA-ITZ NPs, respectively. For L.(L.) infantum there was a reduction (p < 0.001) from 18.1% to 4.8% and 8.3% for PLGA-ITZ-mannose NPs and PLGA-ITZ NPs, respectively. Further with L.(L.) braziliensis amastigotes there was a significant reduction (p < 0.001) from 54.9% to 28% and 21.1% for PLGA-ITZ-mannose NPs and PLGA-ITZ NPs, respectively. Adding mannose increased the efficacy PLGA-ITZ NPs against L.(L.) infantum, while it had no effect against L(V.) panamensis and L.(L.) braziliensis amastigotes. We recommend further investigation of PLGA-ITZ-mannose NPs in animal models to evaluate their potential. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 00B: 000-000, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 680-687, 2019. |
| Databáze: | OpenAIRE |
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