HIGH YIELD PURIFICATION OF DEXTRANSUCRASE FROM LEUCONOSTOC MESENTEROIDES NRRL B-512F BY PHASE PARTITIONING
| Autor: | Manisha Nigam, Sarvagya S. Katiyar, Arun Goyal |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pharmacology
PEG 400 Sucrose Chromatography biology technology industry and agriculture Biophysics macromolecular substances Cell Biology Dextransucrase activity biology.organism_classification Dextransucrase chemistry.chemical_compound Dextran chemistry Biochemistry Leuconostoc mesenteroides Yield (chemistry) PEG ratio Food Science |
| Zdroj: | Journal of Food Biochemistry. 30:12-20 |
| ISSN: | 1745-4514 0145-8884 |
| DOI: | 10.1111/j.1745-4514.2005.00047.x |
| Popis: | The extracellular dextransucrase (sucrose: 1,6-α-D-glucan 6-α-D-glucosyltransferase EC 2.4.1.5) from Leuconostoc mesenteroides NRRL B-512F was purified by phase partitioning using poly(ethylene) glycol (PEG) and dextran generated by dextransucrase. Three steps of repeated phase partitioning by PEG 6000 and PEG 400 showed that the purification of dextransucrase by PEG 6000 was much greater than that obtained by PEG 400. Both the specific activity of 42.1 U/mg and the overall yield of 84% of dextransucrase activity obtained after the third step of phase partitioning by PEG 6000 were significantly higher than that of 23.8 U/mg and 46% overall yield, respectively, by PEG 400 or of that previously reported. The successive three steps, two-phase partitioning with a final concentration of 5% PEG 6000, reproducibly yielded a homogeneous preparation of dextransucrase. |
| Databáze: | OpenAIRE |
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