Presence of Clustered GM1 Ganglioside in the Membrane of Endometrial Mesenchymal Stem Cells is Dependent on Cell Cycle Stage

Autor: Anastasia V. Sudarikova, Elena A. Morachevskaya, Yu. A. Negulyaev, V. I. Chubinskiy-Nadezhdin, Olga G. Lyublinskaya, M. A. Shilina
Rok vydání: 2021
Předmět:
Zdroj: Cell and Tissue Biology. 15:120-126
ISSN: 1990-5203
1990-519X
DOI: 10.1134/s1990519x21020024
Popis: Gangliosides, representing the group of sialic glycosphingolipids, are typical components of lipid rafts of cell membranes and play an important role in the processes of reception and signal transduction. Gangliosides attract specific attention as possible regulators of directed differentiation of mesenchymal stem cells (MSCs). GM1 ganglioside is considered as a marker of lipid microdomains, and fluorescence labeling of clusters of GM1 molecules is one of the main methods for assessing the integrity or destruction of rafts in various types of cells. At the same time, data on the presence and possibility of determining GM1 ganglioside in the plasma membrane of MSCs are extremely limited. The objective of this work was to identify the ganglioside GM1 in the membrane of human endometrial MSC (eMSC) using an experimental approach based on interaction with the pentamer of the beta subunits of cholera toxin (CTB). Fluorescence staining of cells using FITC-CTB conjugate revealed the heterogeneity of the eMSC culture by the presence of GM1 membrane clusters. Experiments on synchronized culture have shown that the amount of clustered GM1 in the eMSC plasma membrane depends on the phase of the cell cycle: it is maximum when the cells are stopped at the G0/G1 phase boundary and decreases when proliferation starts, reaching a minimum in the G2/M phases. The data suggest the functional role of GM1-containing lipid rafts in cell cycle of eMSCs and possible changes in the mechanical properties of the membrane before cell division.
Databáze: OpenAIRE
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