Protective effect of astaxanthin against cisplatin-induced gastrointestinal toxicity in rats

Autor: Levent Tumkaya, Tolga Mercantepe, Yeliz Yilmaz, Kerimali Akyildiz
Rok vydání: 2020
Předmět:
Zdroj: European Surgery. 54:32-38
ISSN: 1682-4016
1682-8631
DOI: 10.1007/s10353-020-00643-2
Popis: The aim of the present study was to demonstrate astaxanthin’s attenuating effects against cisplatin (CIS)-induced gastrointestinal toxicity in a rat model. Thirty-two Wistar rats (weight 200–250 g) were divided into four groups (8 rats/group): control group, CIS group, astaxanthin 25 (ASTA25) group, and astaxanthin 75 (ASTA75) group. Tissue samples of small intestine extracted from rats were histopathologically and immunohistochemically analyzed. Histopathological findings were graded with the Duodenal Histopathological Damage Score. Evaluation of enterocytes showing caspase‑3 positivity was performed using the Immunohistochemistry Positivity Score. Intestinal glutathione (GSH) shows the endogenous antioxidant level. Lipid peroxidation in the intestinal tissue was evaluated by measuring the level of malondialdehyde (MDA), which is the end product of lipid peroxidation. In the ASTA25 group, we observed a significant decrease in the number of necrotic enterocytes, mucosal ulceration, and inflammation. In the ASTA75 group, enterocytes with normal microvilli were noticeable. According to the immunohistochemical results, in the ASTA25 and ASTA75 groups, caspase‑3 positivity scores in enterocytes were lower than the CIS group. Astaxanthin administration significantly inhibited MDA increase in intestinal tissue (p
Databáze: OpenAIRE
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