Electrochemically controlled binding of ethidium to calf thymus DNA at a carbon-paste / ethidium tetracyanoquinodimethane electrode

Autor:	James Q. Chambers, R.D. Mounts, Mark A. Lange, David S. Trimble
Rok vydání:	1989
Předmět:	chemistry.chemical_compound Aqueous solution chemistry Intercalation (chemistry) Electrode Fluorescence spectrometry Analytical chemistry Cyclic voltammetry Electrochemistry Tetracyanoquinodimethane DNA Nuclear chemistry
Zdroj:	Journal of Electroanalytical Chemistry and Interfacial Electrochemistry. 266:277-285
ISSN:	0022-0728
Popis:	Ethidium tetracyanoquinodimethane, Et-TCNQ, carbon paste composite electrodes have been fabricated and studied in contact with aqueous solution. Low current efficiencies for the potential-dependent release of ethidium cations from the electrode surface were observed. In the presence of calf thymus DNA in solution, potential-dependent intercalation of ethidium from the electrode surface was demonstrated by detection of the fluorescence of the intercalated ion. The maximum fluorescence intensity was linear with DNA concentration over the range from 3 to 150 ppm calf thymus DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1b86a88b54ef61fc68bccc2251a51c28 https://doi.org/10.1016/0022-0728(89)85074-0 Zobrazit plný text záznamu