Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
| Autor: | Ugrappa Nagalakshmi, Savithramma P. Dinesh-Kumar, Maria Elena Gamo, Daniel F. Voytas, Pin jui Huang, Evan E. Ellison |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics biology Cas9 Mutant food and beverages RNA RNA virus Plant Science Agrobacterium tumefaciens biology.organism_classification 01 natural sciences Virus 03 medical and health sciences 030104 developmental biology Genome editing Guide RNA 010606 plant biology & botany |
| Zdroj: | Nature Plants. 6:620-624 |
| ISSN: | 2055-0278 |
| Popis: | An in planta gene editing approach was developed wherein Cas9 transgenic plants are infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are augmented with sequences that promote cell-to-cell mobility. Mutant progeny are recovered in the next generation at frequencies ranging from 65 to 100%; up to 30% of progeny derived from plants infected with a virus expressing three sgRNAs have mutations in all three targeted loci. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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