Detection of bacterial contamination, including Bacillus spores, in dry growth media and in milk by identification of their 16S rDNA by polymerase chain reaction

Autor:	Cathrin Koch, Fritz Lembke, Erko Stackebrandt, Jens Sperveslage
Rok vydání:	1996
Předmět:	Microbiology (medical) Sphingomonas paucimobilis biology fungi Bacillus subtilis medicine.disease_cause biology.organism_classification Microbiology Spore Sphingomonas parapaucimobilis medicine Molecular Biology Escherichia coli Incubation Ribosomal DNA Bacteria
Zdroj:	Journal of Microbiological Methods. 26:219-224
ISSN:	0167-7012
DOI:	10.1016/0167-7012(96)00842-1
Popis:	A method for the rapid and reliable detection of Escherichia coli, Sphingomonas parapaucimobilis and Bacillus subtilis in growth media as well as spores of Bacillus species in growth media and milk is introduced. Cells from overnight cultures were added to dry growth media and incubated for several time periods. Plate counts and polymerase chain reaction (PCR) amplification of 16S rDNA were performed after each selected incubation time. No culturable Escherichia coli and Sphingomonas parapaucimobilis cells were detected after 6 days of incubation on dry medium although the total cell number remained constant. In contrast PCR products of 16S rDNA were obtained at each time of the experiment. Presence of spores in dry growth media and milk could be detected after spore activation using a standard PCR assay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1b703775f97f4e8098fe4b180701b80c https://doi.org/10.1016/0167-7012(96)00842-1 Zobrazit plný text záznamu Full Text from ScienceDirect