Isolation of lignocellulose-derived sugars, co-fermentation of lactic acid bacteria strains, and evaluation of L-lactic acid productivity
| Autor: | Yue Shi, Jianguo Liu, Hui Zou, Zhi-qiang Su, Zhan-ying Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering biology food and beverages Bioengineering Cellobiose Lactobacillus pentosus Xylose biology.organism_classification 01 natural sciences Enterococcus faecalis Microbiology Lactic acid 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry 010608 biotechnology Fermentation Waste Management and Disposal Bacteria Enterococcus faecium |
| Zdroj: | BioResources. 12:7859-7872 |
| ISSN: | 1930-2126 |
| DOI: | 10.15376/biores.12.4.7859-7872 |
| Popis: | High-productivity lactic acid bacteria (LAB) strains were screened and their capability to ferment lignocellulose-derived sugars into lactic acid were evaluated. Fifteen LAB strains were successfully isolated from cow dung, haystack, and sheep manure, respectively. Four relatively good strains were selected based on Gram stain, colony morphology, and catalase activity tests. The four strains and commercial inoculants (Lactobacillus pentosus and Enterococcus faecalis) were used to ferment cellobiose/ glucose/xylose to produce high-purity L-lactic acid. One of the strains (N4) presented the highest production of L-lactic acid after fermentation for 12 h and showed a L-lactic acid production of 15.1 g/L, 18.5 g/L, and 2.8 g/L and a productivity of 1.01 g∙L−1∙h−1, 3.68 g∙L−1∙h−1, and 0.47 g∙L−1∙h−1 by metabolizing cellobiose, glucose, and xylose, respectively. Through a phylogenetic tree analysis, strain N4 was identified as Enterococcus faecium and named Enterococcus faecium N4. Enterococcus faecium N4 has a great potential to ferment lignocellulose-derived sugars into L-lactic acid. |
| Databáze: | OpenAIRE |
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