Monoclonal and polyclonal antibodies compared for radioimmunoassay of somatomedin-C in patients with acromegaly or hypopituitarism
| Autor: | T. H. Yeo, P S Sharp, J M Burrin, J L Paterson |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Antiserum
medicine.medical_specialty biology medicine.drug_class business.industry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Hypopituitarism medicine.disease Monoclonal antibody Somatomedin Endocrinology Polyclonal antibodies Internal medicine Monoclonal Acromegaly medicine biology.protein business |
| Zdroj: | Clinical Chemistry. 33:1593-1596 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/33.9.1593 |
| Popis: | We used a synthetic recombinant analog of somatomedin-C (Sm-C) to directly compare the performance of polyclonal and monoclonal antibodies for measuring Sm-C in serum of normal persons and in acromegalic or hypopituitary patients. Mean concentrations of Sm-C in healthy adults were 181 (SD 42) micrograms/L as measured with the monoclonal antibody, 194 (SD 61) micrograms/L with the polyclonal antibody. Both antisera gave excellent discrimination between acromegalics and normals. However, the assay with the polyclonal antibody was more sensitive than that with the monoclonal antibody (lower detection limits: 5 vs 100 micrograms/L) and thus better suited for quantifying Sm-C in samples from hypopituitary patients. |
| Databáze: | OpenAIRE |
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