| Popis: |
Bacterial sigma factors confer promoter specificity to transcription initiated by the RNA polymerase holoenzyme. The alternative sigma factor σ54 (RpoN, NtrA, GlnF) encoded by the rpoN gene allows cells to respond to many different environmental stimuli. Although it is clear that σ54 fulfills a central role during symbiosis, little is known on the regulation of rpoN transcription and activity of its gene product. We have isolated, sequenced and mutated the rpoN gene region of Rhizobium etli, the nodulating symbiont of the common bean plant, Phaseolus vulgaris. |
