| Autor: |
He-Ping Yan, Jucheng Zhang, Lingmei Li, Yun Huang, Yongzhen Feng, Mengting Yang, Wei Liu |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
SPIE Proceedings. |
| ISSN: |
0277-786X |
| Popis: |
The synchronous fluorescence (SF) technique can provide some useful information with the endogenous fluorophores in complex systems. The SF technique has been used in the characterization of the type I collagen in PBS solution (pH=7.4) and the photo-reaction of hypocrellin B (HB) with type I collagen in solution irradiation with the 475 nm light under saturated by oxygen, air and nitrogen respectively. The SF spectra show the peaks attribute to tyrosine residues (275 nm) and pyridinoline cross-link (325 nm) in collagen. The photo-induced reaction with HB causing the fluorescence quenching but no wavelength shift, this suggests the photo-reaction don’t changing the microenvironment of PYD cross-link. The fluorescence quenching rate is faster than others when the solution saturated by oxygen. The results imply that the photo-induced reaction is oxygen dependence. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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