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Figure S4. Phylogenic tree of the putrescine methyltransferase (a), cytochrome P450 nicotine N-demethylase (b), and glutamine synthetase (c) proteins of N. rustica, N. tabacum and their respective progenitors, and their gene expressions, in FPKMs, in various tissues under different growth conditions. For each gene, the relative expressions are highlighted in shades of grey. Sequences for N. tabacum PMT1 (Q42963), PMT2 (Q9SEH7), PMT3 (Q9SEH5), PMT4 (Q9SEH4), CYP82E1 (Q9ZWK2), CYP82E2 (Q38Q85), CYP82E3 (Q38Q84), CYP82E4 (L7Y094), CYP82E5 (A1XEH1), CYP82E8 (A1XEM0), CYP82E10 (E5G962) were obtained from Uniprot. LL: lower leaf, UL: upper leaf, RO: root, FL: flower, GH_1: greenhouse pre-flowering, FI: field pre-flowering, and GH_2: greenhouse flowering. The numbers at each node of the tree correspond to the bootstrapping confidence level of each split. The gene names are composed of the abbreviated progenitor species name, the gene symbol, and the start and stop positions of the BLAST algorithm-based match expressed as a fraction of the protein length. (PDF 485 kb) |
