[OP.2D.03] PULSATILE STRETCH ALTERS EXPRESSION AND PROCESSING OF AMYLOID PRECURSOR PROTEIN IN HUMAN CEREBRAL ENDOTHELIAL CELLS
| Autor: | Vivek Gupta, Roger S. Chung, Sumudu V. S. Gangoda, Alberto Avolio, Mark Butlin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Physiology Pulsatile flow Hemodynamics 02 engineering and technology 010402 general chemistry Bioinformatics 01 natural sciences chemistry.chemical_compound Internal medicine Glyceraldehyde Internal Medicine Amyloid precursor protein Medicine Secretion biology business.industry 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Blot Endocrinology chemistry Cell culture Arterial stiffness biology.protein 0210 nano-technology Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of Hypertension. 34:e24 |
| ISSN: | 0263-6352 |
| DOI: | 10.1097/01.hjh.0000491393.79855.fc |
| Popis: | Amyloid β (Aβ) deposition is a hallmark of Alzheimer's disease (AD). Aβ is generated via enzymatic cleavage of amyloid precursor protein (APP). Increased APP expression is associated with elevated amyloid burden. Elevated pulsatility of arterial pressure, as a vascular factor indicative of vascular stiffness, is associated with AD. This study investigates whether increased magnitude of pulsatile stretch of human cerebral microvascular endothelial cells (hCMEC) alters expression and processing of APP.hCMECs were cultured on flexible silicone chambers and subjected to 5%, 10% or 15% cyclic stretch (ShellPA stretch system, Menicon Life Science) for 18 hours at 1 Hz (n = 5-11 for each stretch magnitude). Comparison was made with controls (0% stretch) to evaluate the effect of pulsatility on APP. APP expression was quantified using western blots with glyceraldehyde 3-phosphate dehydrogenase as the loading control. APP processing was evaluated in the cell culture supernatants by ELISA for secreted Aβ42. Results were analyzed using one way ANOVA (mean ± SEM, % control) or Pearson correlation.The densitometric analysis (expressed as a percentage of control values) of protein expression of APP showed that it was significantly higher at 10% of cyclic stretch (160.9 ± 21%) compared to both the static control (100%, p |
| Databáze: | OpenAIRE |
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