Development, Characterization, and Application of a Versatile Single Particle Detection Apparatus for Time-Integrated and Time-Resolved Fluorescence Measurements—Part II: Experimental Evaluation

Autor:	Xihong Wu, J. A. Merten, N. Omenetto, B. W. Smith, J. D. Winefordner
Rok vydání:	2009
Předmět:	Biochemistry Spectroscopy Atomic and Molecular Physics and Optics
Zdroj:	Laser Chemistry. 2009:1-14
ISSN:	1476-3516 0278-6273
DOI:	10.1155/2009/474858
Popis:	This paper describes the experimental realization and characterization of a versatile single particle detection apparatus. The system utilizes a novel particle beam inlet that can serve as either an on-line particle concentrator (i.e., all diameters confined in a narrow beam) or as a segregator (i.e., selected diameters confined in a narrow beam) and can be operated in a high-speed mode as well as in a low-speed mode, thus allowing different interaction times between the particles and the laser beam. An aerodynamic sizing technique has been incorporated into the system to provide rapid, real-time, and high-resolution sizing. Parameters such as transmission efficiency and size-segregation efficiency have been measured. The performance of the instrument has been demonstrated by on-line detection of spectrally resolved and time resolved fluorescence detection from airborne dye-doped particles and aerosolized endogenous fluorophores found in biological agents.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1affe9a663c0a058386a9ff3d8a4d1af https://doi.org/10.1155/2009/474858 Zobrazit plný text záznamu Plný text ve formátu PDF