Lineal DNA logic gate for microRNA diagnostics with strand displacement and fluorescence resonance energy transfer

Autor:	Jiao-Bao Long, Xiangxian Meng, Ying-Xin Liu, Shu-Ya Yan, Meng-Tan Liu, Tian-Tian Meng, Qing-Feng Cao
Rok vydání:	2015
Předmět:	Chemistry Molecular logic gate fungi Nanotechnology General Chemistry GeneralLiterature_MISCELLANEOUS humanities Displacement (vector) chemistry.chemical_compound Förster resonance energy transfer stomatognathic system Logic gate Work unit Biological system AND gate DNA Hardware_LOGICDESIGN
Zdroj:	Chinese Chemical Letters. 26:1179-1182
ISSN:	1001-8417
DOI:	10.1016/j.cclet.2015.05.039
Popis:	Designing molecular logic gates to operate programmably for molecular diagnostics in molecular computing still remains challenging. Here, we designed a novel linear DNA logic gates for microRNA analysis based on strand displacement and fluorescence resonance energy transfer (FRET). Two labeled strands closed each other produce to FRET through hybridization with a complementary strand to form a basic work unit of logic gate. Two indicators of heart failure (microRNA-195 and microRNA-21) were selected as the logic inputs and the fluorescence mode was used as the logic output. We have demonstrated that the molecular logic gate mechanism worked well with the construction of YES and AND gates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1af7e1cf25f65cb3d592c80b57bb1c9e https://doi.org/10.1016/j.cclet.2015.05.039 Zobrazit plný text záznamu Full Text from ScienceDirect