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Background Brucellosis is a bacterial zoonotic disease that affects animals and humans. Its transmission to humans can occur through various routes such as consumption of infected animal products or unprotected close contact with secretions or different parts of live or dead infected animals. This study aims to report Iranian livestock breeders’ awareness, attitude, practice, and behavioral determinants related to Brucellosis prevention. Methods A cross-sectional study was conducted in 2019 among 450 livestock breeders in Beyraq, a suburb of Tabriz metropolitan city. The Brucellosis Prevention Questionnaire (BPQ) was used to collect data, with statistical analysis performed using SPSS-23. The BPQ, consisting of 53 items, had acceptable psychometric properties (Content Validity Index = 0.90, Content Validity Ratio = 0.74, Impact Score = 4.30, Intra-class Correlation Coefficient = 0.885, Composite Reliability = 0.895, and Standard Error of Measurement = 5.448). Results The study included 450 livestock breeders with a mean age of 51.68 ± 16.4 years and a mean household size of 6.47 ± 2.25. Participants who had a previous history of Brucellosis reported an average occurrence of 7.03 ± 5.83 years ago. Livestock breeders had moderate knowledge levels (mean score = 17.13) and positive attitudes (mean score = 3.86) towards Brucellosis prevention, but their practice level was relatively low (mean score = 15.9). Significant differences were observed in awareness (p-value |
