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Background The lockdown and social distancing caused by COVID-19 may influence common healthy behaviour. Due to the unprecedent worldwide confinement in which Spain has been one of the most affected with severe rules governing confinement, physical activity and sedentarism may change due to prolonged stays at home. The aim of this study is to evaluate how physical activity and sedentary time have changed during confinement in the Spanish population. Methods 3800 healthy adults (age ≥ 18 - ≤64 years) residing in Spain were asked during confinement. Sociodemographic data and self-reported physical activity data were collected twice by an International Physical Activity Questionnaire-Short (IPAQ-S) between 23rd March and 1st April, and the analysis was carried out considering the achievement of general physical activity recommendations, different age ranges and baseline physical activity levels. Results Physical activity decreased significantly during confinement in all population. Vigorous activities and walking time decreased by 16.8% (p-value > 0.001) and 58.2% (p-value > 0.001) respectively, whereas sedentary time increased by 23.8% (p-value > 0.001). The fulfilment of 75 min/week of vigorous activity recommendation decreased by 10.7% (p-value 0.001). Men reduced more the time in vigorous activity than women (21% vs 9%, respectively) who even increased time in moderate physical activity by 11% (p-value |