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Introduction Obesity is a multifactorial disease with genetic, psychological, nutritional, biochemical, and microbiological causes. In previous studies, obesity has been linked to a different composition of the gut microbiota. The gut microbiome composition might be described by several alpha diversity metrics: OTU (operational taxonomic unit) number, Chao1 and ACE (abundance-based coverage estimators), and Shannon and Simpson indices (richness and evenness estimators). On the other hand, recent studies demonstrate a correlation of elevated serum biochemical markers with obesity. Consequently, the linkage between biochemical markers and diversity indices of gut microbiome in obesity might be important and indicative. So, the aim of this study is to check correlation between alpha diversity metrics and obesity-associated metabolic risk factors, such as serum alanine transaminase (ALT) and serum alkaline phosphatase (AP), at patients with obesity and normal weight. Methods Sixteen subjects were obese adolescents (BMI=33.07±4,03 kg/m2), 19 subjects were healthy adolescents (BMI=20.18±1,76 kg/m2). These two groups were comparable by ethnicity (100% participants are Caucasians), gender (20 males, 15 females), and age (mean age 14.7±1.69 years). Metagenome sequencing of V3–V4 regions of 16S rDNA, raw data analysis, OTU clustering, species annotation, and estimation of alpha diversity indices were done by Novogene Company (China). Concentration of serum ALT and AP were measured using Mindray Automatic Biochemistry Analyzer. Data were analyzed using the STATISTICA 8.0 software package. Statistical significance was accepted at the p Results AP data from 11 obese patients, and 7 adolescents with normal weight were included in this study. ALT and OTU number, Chao1, ACE, Shannon and Simpson indices were measured for all respondents (n=35). The Wilcoxon-Mann-Whitney test didn’t show significant differences between the obesity and control groups, despite the fact that alpha diversity metrics and AP were higher in the control group (p>0.05). However, ALT was higher, but not significant in the obesity group (p>0.05). Spearman’s rank correlation coefficient showed significant correlations for AP and ACE и Chao1 (rs=0.93) in the obesity group, but not with Shannon and Simpson indices (rs=0.26).The strong positive correlation also were observed for AP and OTU number (rs=0.85). Either, we observed small, but significant correlations between ALT and ACE, Chao1 и OTU number (rs=0.4). There were no significant relationships between ALT and AP with alpha diversity metrics in the control group (rs≤0.3). Conclusion The study shows a positive relationship between alpha diversity metrics of intestinal microbiota and serum AP and ALT in adolescents with obesity. |
