The glycerophospholipid inventory ofPseudomonas putidais conserved between strains and enables growth condition-related alterations
| Autor: | Andreas Schmid, Lars M. Blank, Heiko Hayen, Jana Rühl, Eva-Maria Hein |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Phosphatidylethanolamine Strain (chemistry) Pseudomonas Fatty acid Bioengineering Glycerophospholipids Biology biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Pseudomonas putida chemistry.chemical_compound Membrane chemistry Glycerophospholipid Biotechnology |
| Zdroj: | Microbial Biotechnology. 5:45-58 |
| ISSN: | 1751-7915 |
| DOI: | 10.1111/j.1751-7915.2011.00286.x |
| Popis: | Summary Microorganisms, such as Pseudomonas putida, utilize specific physical properties of cellular membrane constituents, mainly glycerophospholipids, to (re-)adjust the membrane barrier to environmental stresses. Building a basis for membrane composition/function studies, we inventoried the glycerophospholipids of different Pseudomonas and challenged membranes of growing cells with n-butanol. Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid species [e.g. phosphatidylethanolamine PE(32:1)] with up to five fatty acid combinations were detected. The glycerophospholipid inventory consists of 305 distinct glycerophospholipids [e.g. PE(16:0/16:1)], thereof 14 lyso-glycerophospholipids, revealing conserved compositions within the four investigated pseudomonads P. putida KT2440, DOT-T1E, S12 and Pseudomonas sp. strain VLB120. Furthermore, we addressed the influence of environmental conditions on the glycerophospholipid composition of Pseudomonas via long-time exposure to the sublethal n-butanol concentration of 1% (v/v), focusing on: (i) relative amounts of glycerophospholipid species, (ii) glycerophospholipid head group composition, (iii) fatty acid chain length, (iv) degree of saturation and (v) cis/trans isomerization of unsaturated fatty acids. Observed alterations consist of changing head group compositions and for the solvent-sensitive strain KT2440 diminished fatty acid saturation degrees. Minor changes in the glycerophospholipid composition of the solvent-tolerant strains P. putida S12 and Pseudomonas sp. VLB120 suggest different strategies of the investigated Pseudomonas to maintain the barrier function of cellular membranes. |
| Databáze: | OpenAIRE |
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