Isolation of phospholipase A2 from the venom of the green toadBufo viridis
| Autor: | M. S. Tashmukhamedov, Rakhimov Mm, I. T. Yakubov, Sh. I. Salikhov, Sh. Ya. Mirzaakhmedov, O. K. Zakirova |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Sorbent Chromatography biology food and beverages Venom Plant Science General Chemistry Toad Phosphatidylserine General Biochemistry Genetics and Molecular Biology Hydrolysis chemistry.chemical_compound Enzyme Phospholipase A2 Biochemistry chemistry Covalent bond biology.animal biology.protein |
| Zdroj: | Chemistry of Natural Compounds. 30:97-100 |
| ISSN: | 1573-8388 0009-3130 |
| DOI: | 10.1007/bf00638430 |
| Popis: | Phospholipase A2 has been isolated fromthe venom of he Central Asian green toad Bufo viridis in the electroophoretically homogeneous state for the first time by biospecific absorption chromatography. The affinity sorbent used was Affi-Gel 10 with covalently bound phosphatidylserine (PDS). The chemical inertness of the bound PDS to the hydrolytic action of phospholipase A2 and its high tendency to form biospecific complexes have been shown. Some biochemical characteristics of the enzyme isolated have been studied: influence of the pH and of the Ca2+ concentration and dependence of activity on substrate concentration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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