Autor: | Marc Lefebvre, Kenneth Richards, Laurence Janssens, Hubert Guilley, Emmanuelle Lauber, Gérard Jonard, Guy Weyens |
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Rok vydání: | 2001 |
Předmět: |
0106 biological sciences
0303 health sciences biology Point mutation Mutant Dominant-Negative Mutation biology.organism_classification 01 natural sciences Benyvirus Virology Conserved sequence 03 medical and health sciences Genetics Beet necrotic yellow vein virus Animal Science and Zoology Replicon Agronomy and Crop Science Gene 030304 developmental biology 010606 plant biology & botany Biotechnology |
Zdroj: | Transgenic Research. 10:293-302 |
ISSN: | 0962-8819 |
Popis: | Point mutations were introduced into the genes encoding the triple gene bock movement proteins P13 and P15 of beet necrotic yellow vein virus (BNYVV). Mutations which disabled viral cell-to-cell movement in Chenopodium quinoa were then tested for their ability to act as dominant negative inhibiters of movement of wild-type BNYVV when expressed from a co-inoculated BNYVV RNA 3-based replicon. For P13, three types of mutation inhibited the movement function: non-synomynous mutations in the N- and C-terminal hydrophobic domains, a mutation at the boundary between the N-terminal hydrophobic domain and the central hydrophilic domain (mutant P13-A12), and mutations in the conserved sequence motif in the central hydrophilic domain. However, only the “boundary” mutant P13-A12 strongly inhibited movement of wild-type virus when expressed from the co-inoculated replicon. Similar experiments with P15 detected four movement-defective mutants which strongly inhibited cell-to-cell movement of wild-type BNYVV when the mutants were expressed from a co-inoculated replicon. Beta vulgaris transformed with two of these P15 mutants were highly resistant to fungus-mediated infection with BNYVV. |
Databáze: | OpenAIRE |
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