Study of the efficacy of N-methyl glucamine antimoniate (SbV) associated with photodynamic therapy using liposomal chloroaluminium phthalocyanine in the treatment of cutaneous leishmaniasis caused by Leishmania (L.) amazonensis in C57BL6 mice
| Autor: | Jefferson Bruno Pereira Ribeiro, Jonathan Rosa Moreira, Alexandre Vasconcelos Lima, Gabriela Mariângela Farias de Oliveira, Ana Luisa Miranda-Vilela, Gustavo Henrique Soares Takano, Izabel Cristina Rodrigues da Silva, Rafaela Debastiani Garcia, Ciro Martins Gomes, Raimunda Nonata Ribeiro Sampaio, Ana Angélica Santarem Amorim |
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| Rok vydání: | 2019 |
| Předmět: |
biology
business.industry medicine.medical_treatment Biophysics Photodynamic therapy Dermatology Drug resistance Pharmacology Leishmania biology.organism_classification medicine.disease Oncology Cutaneous leishmaniasis In vivo medicine Parasite hosting Pharmacology (medical) Adverse effect business Amastigote |
| Zdroj: | Photodiagnosis and Photodynamic Therapy. 26:261-269 |
| ISSN: | 1572-1000 |
| Popis: | Background Pentavalent antimonials remain first-line drugs in the treatment of cutaneous leishmaniasis (CL); however, adverse effects and drug resistance have led to the search for less toxic and more effective treatments. As an alternative, topical phthalocyanine has been studied and its efficacy and low toxicity demonstrated. We aimed to study the in vivo efficacy of N-methyl glucamine antimoniate (NMG) associated with photodynamic therapy (PDT) with topical liposomal chloroaluminium phthalocyanine (AlClPC) in the treatment of experimental CL by L. amazonensis. Methods Experimental study with 54 C57BL6 isogenic mice divided into 9 groups including uninfected control, untreated control, PDT with AlClPC + NMG at doses of 10 and 20 mgSbV/Kg/day. The criteria to evaluate the treatment efficacy were: paw diameter, amastigote count, culture, viability test and parasite counts using MTT (3-bromo-4,5-dimethylthiazol-2,5-diphenyl-tetrazolium bromide). Results Treatment of CL with the association of NMG20 + PDT with AlClPC showed significant reduction of paw diameter, amastigote count, cultures, viability test and parasite counts. Parasite reduction occurred at the 10th and 20th days of treatment and 60 days after treatment ended, indicating that parasites did not multiply again. The NMG10 + PDT group with AlClPC presented results equivalent to gold-standard treatment (20 mgSbV/kg/day). Biochemical and histopathological evaluation showed minor changes. Conclusion Treatment of CL caused by L. amazonensis with NMG20 mgSbV/kg/day + PDT with AlClPC was more effective than the traditional NMG20 mgSbV/kg/day. |
| Databáze: | OpenAIRE |
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