Preservation of β-Galactosidase Transgene in Decalcified Murine Bone Specimens Embedded in Paraffin
| Autor: | Pamela Dann, Melissa A. Kacena, Nancy Troiano, John J. Wysolmerski, Julie R. Hens |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Journal of Histotechnology. 31:61-64 |
| ISSN: | 2046-0236 0147-8885 |
| Popis: | Pamela Dannl, Julie R. Hensl, Nancy W. Troiano2, John J. Wysolmerskil, and Melissa A. Kacena2,3*I Department of Internal Medicine, Yale University School of Medicitte, New Haven, CT2 Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, CT" Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, INAbstractGenetically altered mice are an important tool for use inbiomedical research. Several transgenic mice have been cre-ated whereby activation of the transgene results in the pro-duction of B-galactosidase, which can be detected with theuse of histochemical methods. Although good visualizationof enzyme activity in soft tissue can be complicated, it isparticularly difficult in bone specimens, especially thosethat have been decalcified. For example, recent studies havedemonstrated the importance of canonical Wnt signaling inbone. Tcf optimal promoter B-galactosidase (TOPGAL) orWnt-indicator transgenic mice, in which activation of Wntsignaling results in B-galactosidase production, have beenvaluable tools in studying the effects of this signaling path-way in other organs. Thus, it would be helpful to be able todetect B-galactosidase enzymatic activity in bone in thesemice. In these studies, we demonstrate that, in bones takenfrom TOPGAL mice, B-galactosidase is better visualizedwhen bones are decalcified and then stained with X-gal asopposed to being stained with X-gal and then being decal-cified. (The J Histotechnol 3l:6I,2008)Submitted October 22,2007; accepted with revisions March |
| Databáze: | OpenAIRE |
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