Conjugates of Fluorescein and Saenta (5′-S-(2-Aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine): Flow Cytometry Probes for theESNucleoside Transporter Elements of the Plasma Membrane

Autor:	Carol E. Cass, Morris J. Robins, John K. Buolamwini, Alan R. P. Paterson, Wendy P. Gati, James D. Craik, James S. Wiley
Rok vydání:	1994
Předmět:	biology medicine.diagnostic_test Nucleoside transporter Membrane transport Biochemistry Fluorescence Flow cytometry chemistry.chemical_compound Membrane chemistry Genetics biology.protein medicine Fluorescein Molecular probe Nucleoside
Zdroj:	Nucleosides and Nucleotides. 13:737-751
ISSN:	0732-8311
DOI:	10.1080/15257779408013276
Popis:	SAENTA was linked to the C-5 or C-6 positions of fluorescein through several structures to form conjugates that were bound tightly to plasma membrane sites associated with es nucleoside transport activity. The conjugates imparted fluorescence to cells that expressed es nucleoside transport activity and served as es-selective plasma membrane stains suitable for flow cytometry. Prior treatment of es-expressing cells with nitrobenzylthioinosine prevented fluorescent staining with the conjugates. Seven SAENTA-fluorescein conjugates served as flow cytometric stains with high affinities for es sites, despite substantial differences in the SAENTA-fluorescein linkage structures.
Databáze:	OpenAIRE
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