Porins from marine bacteria of the genus Pseudoalteromonas (Gammaproteobacteria: Pseudoalteromonadaceae)

Autor:	Galina N. Likhatskaya, O. Yu. Portnyagina, Lyudmila A. Romanenko, G. M. Frolova, V. A. Khomenko, Olga D. Novikova, T. F. Solovyeva, S. M. Kuznetsova
Rok vydání:	2013
Předmět:	Aquatic Science Biology Oceanography biology.organism_classification General bacterial porin family chemistry.chemical_compound Marine bacteriophage chemistry Biochemistry Gammaproteobacteria Sodium dodecyl sulfate Bacterial outer membrane Lipid bilayer Polyacrylamide gel electrophoresis Bacteria
Zdroj:	Russian Journal of Marine Biology. 39:58-64
ISSN:	1608-3377 1063-0740
DOI:	10.1134/s1063074013010057
Popis:	Outer membrane (OM) fractions were isolated from marine bacteria of the genus Pseudoalteromonas (P. haloplanktis, P. tetraodonis, and Pseudoalteromonas sp. KMM 223). The purity of OM fractions was confirmed by ultracentrifugation in a sucrose gradient. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the bilayer lipid membrane (BLM) technique, heat-modifiable porin-like proteins were identified among the OM proteins of marine pseudomonads. The pore-forming P-1 and P-2 proteins with molecular masses of 43 and 39 kDa, respectively, were obtained from the marine bacterium P. haloplanktis. The nature of current fluctuations in the BLM and the conductivity of pores formed by these proteins suggest that these isolated porins are not identical in their functional properties. A nonlinear dependence of channel conductivity on salt concentration in the aqueous phase was found for the P-2 protein, which is typical of marine bacterial porins.
Databáze:	OpenAIRE
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