Ultrasensitive Fluorescence Determination of 6-Thioguanine in Biological Samples Based on the Silver Nanoparticle-Mediated Release of Acridine Orange Probe
| Autor: | V. K. Verma, Kavita Tapadia, Ashima Sharma, Tungabidya Maharana |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Detection limit Guanine 010401 analytical chemistry Acridine orange 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Acceptor Fluorescence Silver nanoparticle 0104 chemical sciences chemistry.chemical_compound Förster resonance energy transfer chemistry Non-covalent interactions 0210 nano-technology Spectroscopy Nuclear chemistry |
| Zdroj: | Journal of Applied Spectroscopy. 87:357-363 |
| ISSN: | 1573-8647 0021-9037 |
| DOI: | 10.1007/s10812-020-01007-0 |
| Popis: | This article reports a simple, convenient, and very sensitive method for the determination of 6-thio guanine (6-TG). The basic phenomenon in the proposed method is fluorescence resonance energy transfer, where acridine orange (AO) act as a donor and citrate-stabilized silver nanoparticles (AgNPs) as an acceptor. A noncovalent bond between the surfaces of AgNPs and AO was found, and the effect of fluorescence quenching was observed. The fluorescence spectra of AO recovered after further addition of 6-TG are responsible for the aggregation of AgNPs. Under ideal conditions, the linear relationship of 6-TG in the concentration range of 0.005 to 0.04 μM was displayed, and the limit of detection of 6-TG was obtained as 5.3 nM. Under ideal conditions, the linear relationship of 6-TG was displayed in the concentration range of 0.005 to 0.04 μM, and the limit of detection of 6-TG was obtained as 5.3 nM. The proposed method offers a rapid analysis to determine 6-TG in human serum, blood, and urine samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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