No-React Detoxification Process: A Superior Anticalcification Method for Bioprostheses11Presented at the Thirty-second Annual Meeting of The Society of Thoracic Surgeons, Orlando, FL, Jan 29–31, 1996
| Autor: | Amir Abolhoda, Qi Lu, J. Rodrigo Oyarzun, Keith R Allen, John D. Bogden, John R. McCormick, Shenggao Han, Sumei Yu, Shlomo Gabbay, Francis W. Kemp |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pulmonary and Respiratory Medicine
Aortic valve Pathology medicine.medical_specialty business.industry medicine.disease Andrology chemistry.chemical_compound medicine.anatomical_structure chemistry Cell culture Circulatory system medicine Surgery Heart valve Glutaraldehyde Viability assay Cardiology and Cardiovascular Medicine business Fibroblast Calcification |
| Zdroj: | The Annals of Thoracic Surgery. 62:1724-1730 |
| ISSN: | 0003-4975 |
| DOI: | 10.1016/s0003-4975(96)00948-4 |
| Popis: | Background . Glutaraldehyde pretreatment of bioprosthetic heart valves is the major pathogenic factor in their calcific degeneration. This comparative study investigates the merit of the No-React aldehyde detoxification process as an alternative modifier of xenograft tissues. Methods . Glutaraldehyde- and No-React-pretreated porcine aortic valve cusps were implanted subcutaneously in 6-week-old rats (n = 20). At 3, 6, and 14 weeks, randomly selected animals were sacrificed and the explants underwent mineral and morphologic analyses. Glutaraldehyde- and No-React-treated bovine pericardium and porcine aortic valve cusp were incubated in fibroblast cell culture plates. Cell viability was observed under reversed microscope at 6, 24, 48, and 96 hours. Erythrosin B dye exclusion test was used to validate percent cell death. Results . Pretreatment with No-React significantly inhibited calcification of aortic cusp subcutaneous implants throughout the 14-week period (mean tissue Ca 2+ content=1.3 ± 0.7 μg/mg at 14 weeks.) Glutaraldehyde-treated cusps underwent protracted calcification (Ca 2+ content=190.6 ± 89.5 μg/mg; p Conclusions . The No-React detoxification process completely abolishes the cytotoxicity of the xenograft tissue and inhibits calcific degeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|