Extrarenal Expression of 25-Hydroxyvitamin D3-1α-Hydroxylase1
| Autor: | Rosemary Bland, Mary C. Williams, Robert W. McNinch, Paul M. Stewart, Daniel Zehnder, Alexander J. Howie, Martin Hewison |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Kidney biology medicine.diagnostic_test Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry 25-Hydroxyvitamin D3 1-alpha-hydroxylase Biochemistry Enzyme assay Gene product Blot Endocrinology medicine.anatomical_structure Enzyme Western blot chemistry Internal medicine biology.protein medicine Immunohistochemistry |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 86:888-894 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.86.2.7220 |
| Popis: | The mitochondrial enzyme 25-hydroxyvitamin D3-1α-hydroxylase (1α-hydroxylase) plays an important role in calcium homeostasis by catalyzing synthesis of the active form of vitamin D, 1,25-dihydroxyvitamin D3, in the kidney. However, enzyme activity assays indicate that 1α-hydroxylase is also expressed in a variety of extrarenal tissues; recent cloning of cDNAs for 1α-hydroxylase in different species suggests that a similar gene product is found at both renal and extrarenal sites. Using specific complementary ribonucleic acid probes and antisera to 1α-hydroxylase, we have previously reported the distribution of messenger ribonucleic acid and protein for the enzyme along the mouse and human nephron. Here we describe further immunohistochemical and Western blot analyses that detail for the first time the extrarenal distribution of 1α-hydroxylase in both normal and diseased tissues. Specific staining for 1α-hydroxylase was detected in skin (basal keratinocytes, hair follicles), lymph nodes (granulomata), colon... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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