Automated Combinatorial Process for Nanofabrication of Structures Using Bioderivatized Nanoparticles
| Autor: | Dietrich Dehlinger, Dalibor Hodko, Benjamin Sullivan, Michael J. Heller, Sadik C. Esener, Paul D. Swanson |
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| Rok vydání: | 2007 |
| Předmět: |
010302 applied physics
Materials science Fabrication Layer by layer Process (computing) Nanoparticle Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Computer Science Applications Medical Laboratory Technology Nanolithography Fully automated CMOS 0103 physical sciences Self-assembly 0210 nano-technology |
| Zdroj: | JALA: Journal of the Association for Laboratory Automation. 12:267-276 |
| ISSN: | 1535-5535 |
| Popis: | A fully automated electronic microarray control system (Nanochip 400 System) was used to carry out a combinatorial process to determine optimal conditions for fabricating higher order three-dimensional nanoparticle structures. Structures with up to 40 layers of bioderivatized nanoparticles were fabricated on a 400-test site CMOS microarray using the automated Nanochip 400 System. Reconfigurable electric fields produced on the surface of the CMOS microarray device actively transport, concentrate, and promote binding of 40 nm biotin- and streptavidin-derivatized nanoparticles to selected test sites on the microarray surface. The overall fabrication process including nanoparticle reagent delivery to the microarray device, electronic control of the CMOS microarray and the optical/fluorescent detection, and monitoring of nanoparticle layering are entirely controlled by the Nanochip 400 System. The automated nanoparticle layering process takes about 2 minutes per layer, with 10–20 seconds required for the electronic addressing and binding of nanoparticles, and roughly 60 seconds for washing. The addressing and building process is monitored by changes in fluorescence intensity as each nanoparticle layer is deposited. The final multilayered 3D structures are about 2 μm in thickness and 55 μm in diameter. Multilayer nanoparticle structures and control sites on the microarray were verified by SEM analysis. |
| Databáze: | OpenAIRE |
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