Optimal Culture Conditions for Production of Subtilisin-like Protease Inhibitor from Streptomyces thermocarboxydus C12
| Autor: | Jai-Yul Kong, Gyeonglim Choi, Sung-Il Kang, Yeung-Joon Choi, Young-Boo Jang, Byeong-Dae Choi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Nutrition and Dietetics
Chromatography Protease Subtilisin-like protease medicine.medical_treatment Subtilisin Fructose Biology biology.organism_classification Protease inhibitor (biology) chemistry.chemical_compound chemistry Biochemistry Galactose medicine Streptomyces thermocarboxydus Proteose Food Science medicine.drug |
| Zdroj: | Journal of the Korean Society of Food Science and Nutrition. 37:373-378 |
| ISSN: | 1226-3311 |
| DOI: | 10.3746/jkfn.2008.37.3.373 |
| Popis: | The objective of this paper was to investigate optimal culture conditions for the production of an inhibitor against subtilisin-like protease from Streptomyces thermocarboxydus (S. thermocarboxydus) C12 isolated from sediments of Gwangyang coast. The optimal temperature and initial pH for the production of subtilisin-like protease inhibitor were 40 o C and pH 8.0, respectively. Inhibition activities were high for galactose, glucose and fructose. The best carbon source and its concentration were galactose and 1.6% (w/v), respectively. Inhibition activities were also high in medium containing polypeptone, proteose and peptone. Optimal nitrogen source and concentration were protease peptone and 0.5% (w/v), respectively. Optimal concentrations for inhibitor production were 1% (w/v) for NaCl and 1 mM LiCl for metal salts. The subtilisin-like protease inhibitor from S. thermocarboxydus C12 showed a maximum inhibitor activity after cultivation for 84 h under the optimized medium. |
| Databáze: | OpenAIRE |
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