Differences in the Soluble, Residual Phosphate Concentrations at Which Coastal Phytoplankton Species Up-regulate Alkaline-Phosphatase Expression, as Measured By Flow-Cytometric Detection of ELF-97® Fluorescence
| Autor: | Gary H. Wikfors, J. Evan Ward, Shannon L. Meseck, Jennifer H. Alix |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Ecology biology fungi Substrate (chemistry) Aquatic Science Synechococcus biology.organism_classification Phosphate chemistry.chemical_compound Enzyme chemistry Biochemistry Phytoplankton Alkaline phosphatase Phosphorus deficiency Isochrysis Ecology Evolution Behavior and Systematics |
| Zdroj: | Estuaries and Coasts. 32:1195-1204 |
| ISSN: | 1559-2731 1559-2723 |
| DOI: | 10.1007/s12237-009-9211-7 |
| Popis: | The enzyme-labeled fluorescence (ELF-97®) substrate produces an insoluble, fluorescent yellow-green product at the site of alkaline-phosphatase (AP) activity. Fifteen coastal phytoplankton species were tested to determine if AP activity could be detected in phosphate- depleted media. All species tested, except Synechococcus bacillaris, expressed AP activity. Subsequently, threshold concentrations of soluble reactive phosphate (SRP) at which AP activity could be detected by ELF-97® were deter- mined for Chaetoceros neogracile, Chlorella autotrophica, Isochrysis sp., Prorocentrum minimum ,a ndTetraselmis chui. Microalgal species differed significantly in the SRP con- centration at which AP activity was first detectable (10.1- 16.4 µM), well above concentrations normally considered limiting for phytoplankton. P. minimum began to express AP activity at a higher SRP concentration than the other algal species; this may be attributable to a relatively high DNA/cell ratio in P. minimum, compared to the other phytoplankton. Thus, phytoplankton species may respond to phosphorus deficiency at high SRP concentrations. |
| Databáze: | OpenAIRE |
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