Use of Carbon Dioxide- and Oxygen-Tonometered Phosphate— Bicarbonate—Chloride—Glycerol—Water Mixtures for Calibration and Control of pH, pco2 and pO2 Electrode Systems

Autor:	R. A. M. Van den Camp, A. H. J. Maas, A. H. Veefkind
Rok vydání:	1975
Předmět:	Bicarbonate Biochemistry (medical) Clinical Biochemistry Analytical chemistry chemistry.chemical_element Partial pressure Phosphate Chloride Nitrogen Solvent Partition coefficient chemistry.chemical_compound chemistry Carbon dioxide medicine medicine.drug Nuclear chemistry
Zdroj:	Clinical Chemistry. 21:685-693
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/21.6.685
Popis:	Calibration of pH, pco2, and po2 electrode systems of modern blood—gas analyzers, designed with one sample cuvet for measurement, is mostly performed separately with buffer solutions and gas mixtures. We describe the development of solutions of known pH, pco2 and po2 for doing such calibrations simultaneously, containing phosphate, bicarbonate, and chloride in glycerol—water mixtures as solvent. A method is suggested for computing the relation between pH and log pCO2 of these solutions in equilibrium with carbon dioxide gas. It is demonstrated that a solution of phosphate (Na2HPO4, KH2PO4, each 25.mmol/liter), bicarbonate (NaHCO3, 30 mmol/ liter), and chloride (NaCI, 30 mmol/ liter) in glycerol-water mixture (3/7 by vol) and equilibrated with CO2 in air (4 vol/100 vol) and CO2 in nitrogen (8 vol/100 vol), respectively, makes possible accurate and simultaneous calibration of the pH, pco2, po2 electrodes of a Corning Model 165 blood—gas analyzer. Similar solutions may also be used for quality—control of blood—gas measurement.
Databáze:	OpenAIRE
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