| Autor: |
P. Sureau, P. Perrin, D. Portnoï |
| Rok vydání: |
1982 |
| Předmět: |
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| Zdroj: |
Annales de l'Institut Pasteur / Virologie. 133:403-422 |
| ISSN: |
0769-2617 |
| Popis: |
Summary The binding of rabies virus and its entry into susceptible cells was studied using BHK21 cells and a liposomal membrane model of cellular constituents. When BHK21 cells were incubated with radiolabelled virus or purified glycoprotein, binding of viral material to cell surfaces was observed. Saturation of cellular membrane sites by unlabelled rabies glycoprotein did not inhibit binding of radiolabelled virus. This result suggested that the sites and/or the mechanism of binding of the glycoprotein molecules were independant, depending on whether glycoprotein was free in the incubating solution or anchored to the viral membrane. Binding of the virus to the cell membrane and entry of viral material were enhanced by incubating virus-cell mixtures in acidic conditions: the amount of virus radioactivity bound to cells was 10-fold higher at pH 5.4 than at pH 7.2, but the viral infectivity was the same. Furthermore, these two phenomenons required energy because lower amounts of viral radioactivity were detected when virus and cells were incubated at 4°C (instead of 20°C) with sodium azide. Attempts to saturate cell sites with unlabelled virus led to two types of results: (1) inhibition of binding of labelled virus by low concentrations. Rabies virus also bound to liposomes prepared with either cellular lipids alone or lipids and 0.01% Triton-X100 cellular extracts. This binding was specific only at pH 7.2 and only when virus was incubated with liposomes containing detergent extract. Under these conditions, liposomes could be partially saturated when incubated with unlabelled virus before contact with labelled virus. This observation suggests that virus bind to a detergent-soluble receptor. All these results led us to propose that the attachment of rabies virus to the BHK21-cell surface requires a specific receptor and that entry of viral material mainly involves a fusion of the viral membrane and the cell plasma membrane. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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