DEVELOPMENT OF ARGININE DEIMINASE BASED CONDUCTOMETRIC BIOSENSOR FOR ARGININE DETERMINATION

Autor:	I. S. Kucherenko, D. Yu. Kucherenko, S. V. Marchenko, Sergei V. Dzyadevych, A. P. Soldatkin, O. Ya. Sayapina, Oleksandr O. Soldatkin
Rok vydání:	2021
Předmět:	Arginine Biochemistry Chemistry technology industry and agriculture macromolecular substances Biosensor Arginine deiminase
Zdroj:	Sensor Electronics and Microsystem Technologies. 18:4-13
ISSN:	2415-3508 1815-7459
Popis:	For the first time, a conductometric enzyme biosensor was developed to determine arginine concentrations. The bioselective membrane of the biosensor was formed by immobilization of arginine deiminase on the surface of gold planar transducer using covalent crosslinking of glutaraldehyde with bovine serum albumin. An effect of the solution characteristics (ionic strength, buffer capacity) on the biosensor functioning was studied. The proposed monoenzyme biosensor was shown to have high sensitivity to arginine (minimum limit of detection - 5 μM) and good selectivity towards possible interferents. The linear range of determination was from 10 to 800 μM. The biosensor sensitivity to arginine is 72 μS /μM. The developed biosensor was demonstrated to be promising for the arginine analysis in real samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1a42310b05c6abe103ce6aba68b34029 https://doi.org/10.18524/1815-7459.2021.2.235200 Zobrazit plný text záznamu