WITHDRAWN: Detection of human genetic variation in VAC14 gene by ARMA-PCR technique and relation with typhoid fever infection in patients with gallbladder diseases in Thi-Qar province/Iraq
| Autor: | Zaman K. Hanan, Ezat H. Mezal, Manal B. Saleh, Abduladheem Turki Jalil |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
010302 applied physics
medicine.medical_specialty business.industry Incidence (epidemiology) Gallbladder Gallbladder disease Histology 02 engineering and technology Gene mutation bacterial infections and mycoses 021001 nanoscience & nanotechnology medicine.disease 01 natural sciences Gastroenterology Typhoid fever medicine.anatomical_structure Internal medicine 0103 physical sciences medicine Gene polymorphism Gallbladder cancer 0210 nano-technology business |
| Zdroj: | Materials Today: Proceedings. |
| ISSN: | 2214-7853 |
| Popis: | Our study screen for S. Typhi present in the gallbladder of (2 0 0) patients suffering from GD along with 40 normal gallbladders, the samples were taken and diagnosed at the Al-Hussein teaching and Al-Amel hospitals, as well as the Noor Al Hussein and Ibn Al-Baitar laboratories, in Thi-Qar Province, Iraq at the period between February and October 2020, from both sexes, with ages ranging from 13 to 98 years. Histology along with the microbiological and molecular screening of specimens by PCR method for S. Typhi detection in samples. On histopathological observations, the most common GD were cholelithiasis (69%) than acute acalculous cholecystitis (without stones) (18.5%) while (12.5%) were suffering from gallbladder cancer .they were all undergoing clinically and CT examination and PCR assay for screening of S.Typhi in GD patients appeared (13.06%) of them suffering from typhoid fever. Investigation VAC14 gene mutation in GD patients infected with Typhoid fever by gene polymorphism ARMS-PCR technique revealed that 10 (38.46%) cases of GD patients infected with S. Typhi carry a mutation in the VAC14 gene while only 3(11.5%) cases of GD patients not infected with S. Typhi recorded a mutation in VAC14 gene and recorded that GD patients infected with typhoid fever that has about five times the risk of a gene VAC14 mutation than the incidence of the same mutation in GD patients without typhoid fever and the patient infected with typhoid fever that contains cdtB gene have ten times the risk of a gene VAC14 mutation than the incidence of the same mutation in a patient infected with typhoid fever that not contain cdtB gene. |
| Databáze: | OpenAIRE |
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