| Popis: |
PSF engineering has been widely used over the past years to enable 3D localization in Single Molecule Localization Microscopy (SMLM) techniques, such as PALM/STORM superresolution methods. It can make use of a cylindrical lens, a phase mask, a Spatial Light Modulator, a Deformable Mirror (DM) to encode depth on a spatial variation of the PSF along the Z axis. Among these techniques, we demonstrate that a DM-based approach – such as implemented in our MicAO-3DSR system - provides the best versatility/performance combination |
