Tissue culture of crownvetch (Coronilla varia L.) and the production of cardenolide-like substancesin vitro

Autor:	Věra Spurná, Jiřina Dušková, Marie Sovová, Pavla Žáčková
Rok vydání:	1987
Předmět:	Plant Science Horticulture Biology biology.organism_classification Thin-layer chromatography In vitro chemistry.chemical_compound Tissue culture chemistry Coronilla Botany Cardenolide Composition (visual arts) Subculture (biology) Explant culture
Zdroj:	Biologia Plantarum. 29:258-264
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02892786
Popis:	The caryotype (2n = 24) of crownvetch (Coronilla varia L.) and the conditions of successful cultivation of tissue cultures derived from it were determined. The Murashige-Skoog cultivation medium with the addition of indol-3-ylacetic acid (1 mg 11), 1-naphthaleneacetic acid (0.1 mg 11), or coconut milk (20 %) was found to be the most suitable. A complex of cardenolide-like substances identical in its composition with that found in leaves of intact crownvetch plants was detected by means of thin layer chromatography in the extracts of these explant cultures up to the seventh subculture. These findings are also in agreement with the results of our earlier experiments in which strong pharmacological effects of cardenolides obtained from tissue cultures of this plant were demonstrated. Further investigations must be devoted to the optimalization of cultivation conditions of crownvetch cultures with the aim of their utilization as anin vitro source of cardioactive substances.
Databáze:	OpenAIRE
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