Microsatellite markers for the endangered franciscana dolphin (Pontoporia blainvillei)
| Autor: | Nathalia P. Cavaleiro, Haydée A. Cunha, Cristiano Lazoski, Marta Jussara Cremer, Teresa E. C. dos Santos, Adriana C. Colosio, Lupércio A. Barbosa, Luísa C. Alvarenga |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
education.field_of_study Population Endangered species Population genetics Zoology General Medicine Biology Hardy–Weinberg principle Loss of heterozygosity 03 medical and health sciences Phylogeography 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Threatened species Genetics Microsatellite education Molecular Biology |
| Zdroj: | Molecular Biology Reports. 48:3011-3016 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-021-06263-7 |
| Popis: | The franciscana (Pontoporia blainvillei) is the most threatened small cetacean in the South Atlantic. In this study we report the development of 13 microsatellite markers for franciscanas through next-generation sequencing, and the characterization of those loci in 38 samples from the species’ northernmost population (Espirito Santo, Brazil). Besides providing diversity indices for the new, specific loci, we also report on the transferability of heterologous loci which had not been screened in franciscanas before, and review all loci used in previous studies. Expected heterozygosity in the new loci ranged between 0.107 and 0.595, and all but one were in Hardy Weinberg Equilibrium. These are the first microsatellite loci isolated from franciscanas, and they are an important addition to heterologous markers that were available previously. |
| Databáze: | OpenAIRE |
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