Advanced Mg, Zn, Sr, Si Multi-Substituted Hydroxyapatites for Bone Regeneration
| Autor: | Janis Locs, Matteo D'Este, Maria Tomoaia-Cotisel, Corina Garbo, Gerard Demazeau, Cecilia Roman, Ossi Horovitz, Aurora Mocanu |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Simulated body fluid Organic Chemistry Biophysics Pharmaceutical Science Nanoparticle Bioengineering 02 engineering and technology General Medicine 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Nanomaterials Biomaterials Crystallinity Chemical engineering Specific surface area Drug Discovery Hydroxyapatites Crystallite 0210 nano-technology Bone regeneration |
| Zdroj: | International Journal of Nanomedicine. 15:1037-1058 |
| ISSN: | 1178-2013 |
| Popis: | Purpose Compositional tailoring is gaining more attention in the development of advanced biomimetic nanomaterials. In this study, we aimed to prepare advanced multi-substituted hydroxyapatites (ms-HAPs), which show similarity with the inorganic phase of bones and might have therapeutic potential for bone regeneration. Materials Novel nano hydroxyapatites substituted simultaneously with divalent cations: Mg2+ (1.5%), Zn2+ (0.2%), Sr2+ (5% and 10%), and Si (0.2%) as orthosilicate (SiO4 4-) were designed and successfully synthesized for the first time. Methods The ms-HAPs were obtained via a wet-chemistry precipitation route without the use of surfactants, which is a safe and ecologically friendly method. The composition of synthesized materials was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The materials were characterized by X-ray powder diffraction (XRD), FT-IR and FT-Raman spectroscopy, BET measurements and by imaging techniques using high-resolution TEM (HR-TEM), FE-SEM coupled with EDX, and atomic force microscopy (AFM). The ion release was measured in water and in simulated body fluid (SBF). Results Characterization methods confirmed the presence of the unique phase of pure stoichiometric HAP structure and high compositional purity of all synthesized nanomaterials. The doping elements influenced the crystallite size, the crystallinity, lattice parameters, morphology, particle size and shape, specific surface area, and porosity. Results showed a decrease in both nanoparticle size and crystallinity degree, coupled with an increase in specific surface area of these advanced ms-HAP materials, in comparison with pure stoichiometric HAP. The release of biologically important ions was confirmed in different liquid media, both in static and simulated dynamic conditions. Conclusion The incorporation of the four substituting elements into the HAP structure is demonstrated. Synthesized nanostructured ms-HAP materials might inherit the in vivo effects of substituting functional elements and properties of hydroxyapatite for bone healing and regeneration. Results revealed a rational tailoring approach for the design of a next generation of bioactive ms-HAPs as promising candidates for bone regeneration. |
| Databáze: | OpenAIRE |
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