Application of a stability-indicating HPTLC method for quantitative analysis of sertraline hydrochloride in pharmaceutical dosage forms

Autor:	Janhavi R Rao, Mohan Kumar, L. Sathiyanarayanan, Vibha Yadav, Savita S Yadav
Rok vydání:	2011
Předmět:	Detection limit Chromatography Silica gel Clinical Biochemistry Alkaline hydrolysis (body disposal) Biochemistry Dosage form Sertraline Hydrochloride Analytical Chemistry Absorbance chemistry.chemical_compound chemistry Photodegradation Quantitative analysis (chemistry)
Zdroj:	Journal of Planar Chromatography – Modern TLC. 24:140-144
ISSN:	1789-0993 0933-4173
Popis:	A sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of sertraline hydrochloride in the bulk drug and in formulations has been established and validated. Aluminum foil HPTLC plates precoated with silica gel 60F254 as stationary phase were used with toluene-ethyl acetate-ethanol-ammonia 8:2:0.5:0.1 (ν/ν). A compact spot was obtained for sertraline hydrochloride (RF 0.33 ± 0.02). Densitometric analysis was performed in absorbance mode at 273 nm. Regression analysis of calibration data revealed a good linear relationship (r2 = 0.9996 ± 0.02) between response and concentration over the range 2000–12000 ng per band. The method was validated for accuracy, precision, linearity, limits of detection and quantification, and robustness. Sertraline hydrochloride was subjected to acidic and alkaline hydrolysis, oxidation, dry heat treatment, and photodegradation. Peaks of degradation products were well resolved from that of the pure drug and h...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::19f783509b55418e3dcb314cefef124f https://doi.org/10.1556/jpc.24.2011.2.11 Zobrazit plný text záznamu Full text from SpringerLink