| Popis: |
Dogs with vagally innervated gastric pouches (PP), gastric fistulae, and pancreatic fistulae were studied. Added to a background Urecholine infusion (100 μg per kg-hr), caerulein, in doses ranging from 30 to 250 ng per kg-hr, caused an increase of PP response with the maximum reaching about 80% of the maximal response to histamine dihydrochloride (320 μg per kg-hr) and 55% of the maximal response to a meat meal of 25 g per kg. Endogenous cholecystokinin, released by intraduodenal instillation of amino acids, produced pancreatic protein output similar to that obtained with caerulein at the dose of 120 ng per kg-hr, and resulted in an increase of Urecholine-induced acid secretion, reaching about 50% of the maximal response to histamine. After surgical conversion of PP to Heidenhain pouches, the addition of caerulein to a background Urecholine stimulation resulted in dose-related increases in acid output from the Heidenhain pouch, reaching, at the highest side effect free dose of caerulein (250 ng per kg-hr), about 170% of the maximal response to histamine and about 180% of the response to feeding. The highest pancreatic protein outputs in response to various doses of caerulein, or intraduodenal amino acids combined with Urecholine, were significantly higher than those in response to caerulein or amino acids alone, and comparable to those obtained with a meat meal and did not change after transformation of PP to Heidenhain pouch. These results indicate that the combination of cholecystokinin and cholinergic drug results in the increase of gastric acid and pancreatic protein secretion, an effect that is particularly pronounced in vagally denervated gastric pouches. |
