Design strategies for C-phycocyanin purification: Process influence on purity grade
| Autor: | Luisa Sala, Anna Rafaela Cavalcante Braga, Susana Juliano Kalil, Caroline Costa Moraes, Marina Campos Assumpção De Amarante |
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| Rok vydání: | 2020 |
| Předmět: |
Fractional Precipitation
Chromatography Antioxidant Elution Chemistry medicine.medical_treatment Ion chromatography Ultrafiltration Filtration and Separation 02 engineering and technology C-Phycocyanin 021001 nanoscience & nanotechnology Analytical Chemistry Membrane 020401 chemical engineering Scientific method medicine 0204 chemical engineering 0210 nano-technology |
| Zdroj: | Separation and Purification Technology. 252:117453 |
| ISSN: | 1383-5866 |
| Popis: | The performance of four purification designs combining fractional precipitation, ultrafiltration, and ion exchange chromatography (IEC) was compared in the purification of C-phycocyanin (C-PC) from the wet biomass of Spirulina platensis LEB-52. The purification strategies were evaluated in terms of purity grade, purification factor and recovery of the product. Additionally, the antioxidant activities of the clarified and purified C-PC extracts were compared. Initially, three polyethersulfone ultrafiltration membranes of different molecular weight cut-offs (30, 50, and 100 kDa) were evaluated in the purification of C-PC. The 30 and 50 kDa membranes showed statistically equal purification results (p > 0.05), and the 50 kDa membrane was selected to be used in the next steps. Then, different purification designs, which included fractional precipitation, ultrafiltration and IEC were applied. The purification design consisting of ultrafiltration followed by IEC with pH gradient elution was considered the most advantageous because it resulted in C-PC with a purity of 3.9, purification factor of 4.9-fold, and 79.7% recovery. In addition, the antioxidant capacity results showed that the extract with the highest purity (3.9) presented higher scavenging capacity against ABTS●+ (161.66 µmolTE.g−1) and peroxyl (1211.41 µmolTE.g−1) radicals when compared to the clarified extract (8.85 and 29.80 µmolTE.g−1, respectively). This work demonstrates the advantages of applying a purification design to C-PC, as it results in a product of high purification factor, recovery, and antioxidant activity, important characteristics due to the high added-value and biotechnological importance of this biomolecule. |
| Databáze: | OpenAIRE |
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