Two-photon excitation in time-resolved fluorescence microscopy

Autor: Juha Salo, Stefan W. Hell, Erkki Soini, Ari Kuusisto, Lotta Lehtela, Pekka Hänninen
Rok vydání: 1994
Předmět:
Zdroj: SPIE Proceedings.
ISSN: 0277-786X
DOI: 10.1117/12.172107
Popis: Resonant two photon excitation of UV absorbing dyes with red light is a new concept in 3D microscopy. This technique provides functionality which is virtually equivalent to a confocal microscope. Two photon excitation allows excitation of UV dyes without special UV corrected optics. Resonant two photon excitation in microscopy, as known until now, utilizes expensive, high powered pulsed femto- or picosecond lasers. In this paper a cost effective alternative to resonant two photon is presented.© (1994) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Databáze: OpenAIRE