Investigation of DNA Damage Induced by Proton and Gamma Radiation

Autor:	S. V. Paston, D. S. Brozhik, V. F. Ezhov, F. A. Pak, V. N. Verbenko, D. L. Karlin, A. M. Polyanichko, E. V. Chikhirzhina, E. P. Gulevich, A. I. Khalikov, O. M. Kotb
Rok vydání:	2021
Předmět:	Nucleic acid thermodynamics Circular dichroism chemistry.chemical_compound Proton chemistry DNA damage Radiochemistry Biophysics Gamma ray Nucleic acid Irradiation DNA
Zdroj:	Biophysics. 66:202-208
ISSN:	1555-6654 0006-3509
DOI:	10.1134/s0006350921020123
Popis:	In this study, we compared the effects of gamma and high-energy proton radiation (1000 MeV) on DNA in aqueous saline solutions (5 and 150 mM NaCl) at doses of 30 and 50 Gy. We used spectral methods (the ultraviolet absorption method, spectrophotometric methods for nucleic acid quantification, spectrophotometric DNA melting, and circular dichroism) for the estimation of the number of damaged nitrogenous bases and damage of the secondary DNA structure. It was found that under these conditions, proton radiation causes more severe destruction of nitrogenous bases and the secondary DNA structure than exposure to gamma rays at the same dose. In DNA irradiated with protons, the formation of crosslinks is possible and the probability for crosslinking increases with the increase of the ionic strength of the irradiated solution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::19a2790b81b380e61debe32e02a30cca https://doi.org/10.1134/s0006350921020123 Zobrazit plný text záznamu Full text from SpringerLink