Growth of a clonal cell line of Helicoverpa zea (Lepidoptera: Noctuidae) in suspension culture and replication of its homologous baculovirus HzSNPV

Autor:	Arthur H. McIntosh, Cynthia L. Goodman, Carlo M. Ignoffo, James J. Grasela
Rok vydání:	2001
Předmět:	Lepidoptera genitalia Titer biology Inoculation Cell culture Insect Science Botany Noctuidae Doubling time Helicoverpa zea biology.organism_classification Molecular biology Fetal bovine serum
Zdroj:	Applied Entomology and Zoology. 36:349-352
ISSN:	1347-605X 0003-6862
DOI:	10.1303/aez.2001.349
Popis:	A clonal cell line (BCIRL-HZ-AM1-11) of Helicoverpa zea was grown in stationary and suspension cultures in Ex-Cell 401TM medium containing 10% fetal bovine serum at 28°C. The cell population doubling time was 22 h in stationary culture as compared with 27 h in suspension culture. A lag time of approximately 24 h was observed during the first 24 h of the suspension culture following initiation but no lag time was observed in the stationary culture. Maximum viral titers were achieved in stationary and suspension cultures at 120 h (1.80×106 TCID50/ml) and 168 h (1.48×107 TCID50/ml), respectively, following inoculation with the Helicoverpa zea baculovirus (HzSNPV/Br-CL2). Infected cells harvested at 168 h from the 50 ml suspension culture produced a total number of occlusion bodies of 3×109.
Databáze:	OpenAIRE
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